Biochemical differentiation of biotypes
within Prototheca zopfii

 

Assimilation of C- and N- sources by P. zopfii biotypes 1, 2, and 3. 

 

 

 

Auxanographical reactions

 

Nutriment utilization  (BBL Crystal)

 

 

 

GLUd

GLR

GAL

 

GLR

GAL

FGS

INO

TTC

FTR

FVA

ARG

LYS

Biotype

Source

Strain

48a

48

24

48

72

 

48a

48

48

48

48

48

48

48

48

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

1

1

 1

Cow barn

RZ I-1

+++b

+++

(+)

++

+++

 

+ c

+

+

-

+

-

-

+

-

Cow barn

RZ I-2

+++

+++

+

++

+++

 

+

+

+

-

-

+

+

+

-

Cow barn

SAG 2063

+++

+++

+

++

+++

 

+

+

-

+

+

-

+

+

-

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

2

Human enteropathia

SAG 263-4

+++

+++

-

(+)

+

 

+

-

-

-

+

-

-

+

+

2

Bovine mastitis

SAG 2021

+++

+++

-

-

+

 

+

-

+

-

+

+

+

+

+

2

Bovine mastitis

RZ II-1

+++

+++

-

-

(+)

 

+

-

+

-

+

+

+

+

+

Bovine mastitis

RZ II-2

+++

+++

-

(+)

+

 

+

-

+

-

-

+

+

+

+

2

Bovine mastitis

RZ II-3

+++

+++

-

(+)

(+)

 

+

-

+

-

-

-

+

+

+

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3

Piggery

RZ III-1

+++

-

(+)

+

++

 

-

+

-

-

-

-

-

-

-

3

Piggery

RZ III-2

+++

-

(+)

++

++

 

-

+

-

-

-

+

+

-

-

3

Human onychomycosis

SAG 2064

+++

-

(+)

++

+++

 

-

+

-

-

-

-

-

-

-

a Incubation time in hours
b
Intensity of growth: - = no growth, (+) = doubtful, + = weak, ++ = moderate, +++ = strong
c
Nutriment utilization: +  = utilization; - = no utilization
d
C- and N-sources: GLU = glucose, GLR = glycerol, GAL = galactose, FTR = tryptophane, FGS = glucoside, FVA = valine, INO =inositol, 
    TTC = tetrazolium, ARG  = arginine, LYS = lysine

Reference: Uwe ROESLER*, Holger SCHOLZ , Andreas HENSEL (2002): Emended phenotypic charakterization of Prototheca zopfii: A proposal for three biotypes, and standards for their identification. IJSEM Published online 13 December 2002 as http://dx.doi.org/10.1099/ijs.0.02556-0