Biochemical differentiation of biotypes
within Prototheca zopfii

 

Assimilation of C- and N- sources by P. zopfii biotypes 1, 2, and 3. 

 

 

 

Auxanographical reactions

 

Nutriment utilization  (BBL Crystal®)

 

 

 

GLUd

GLR

GAL

 

GLR

GAL

FGS

INO

TTC

FTR

FVA

ARG

LYS

Biotype

Source

Strain

48a

48

24

48

72

 

48a

48

48

48

48

48

48

48

48

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

1

1

 1

Cow barn

RZ I-1

+++b

+++

(+)

++

+++

 

+ c

+

+

-

+

-

-

+

-

Cow barn

RZ I-2

+++

+++

+

++

+++

 

+

+

+

-

-

+

+

+

-

Cow barn

SAG 2063

+++

+++

+

++

+++

 

+

+

-

+

+

-

+

+

-

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

2

Human enteropathia

SAG 263-4

+++

+++

-

(+)

+

 

+

-

-

-

+

-

-

+

+

2

Bovine mastitis

SAG 2021

+++

+++

-

-

+

 

+

-

+

-

+

+

+

+

+

2

Bovine mastitis

RZ II-1

+++

+++

-

-

(+)

 

+

-

+

-

+

+

+

+

+

2 

Bovine mastitis

RZ II-2

+++

+++

-

(+)

+

 

+

-

+

-

-

+

+

+

+

2

Bovine mastitis

RZ II-3

+++

+++

-

(+)

(+)

 

+

-

+

-

-

-

+

+

+

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3

Piggery

RZ III-1

+++

-

(+)

+

++

 

-

+

-

-

-

-

-

-

-

3

Piggery

RZ III-2

+++

-

(+)

++

++

 

-

+

-

-

-

+

+

-

-

3

Human onychomycosis

SAG 2064

+++

-

(+)

++

+++

 

-

+

-

-

-

-

-

-

-

a Incubation time in hours
b Intensity of growth: - = no growth, (+) = doubtful, + = weak, ++ = moderate, +++ = strong
c Nutriment utilization: +  = utilization; - = no utilization
d C- and N-sources: GLU = glucose, GLR = glycerol, GAL = galactose, FTR = tryptophane, FGS = glucoside, FVA = valine, INO =inositol, 
    TTC = tetrazolium, ARG  = arginine, LYS = lysine

Reference: Uwe ROESLER*, Holger SCHOLZ , Andreas HENSEL (2002): Emended phenotypic charakterization of Prototheca zopfii: A proposal for three biotypes, and standards for their identification. IJSEM Published online 13 December 2002 as http://dx.doi.org/10.1099/ijs.0.02556-0