Prototheca blaschkeae
(Roesler et al., 2006)

 

Morphology:

  • yeast like colonies, smooth surface and  smooth margin

  • beige or ocher colored.

  • Micro morphology: round single cells (10 Ám in diameter) and
    some large Sporangia (20-30 Ám) with round endospores inside .

 

Diagnostics

  • plate culture on fungi media, e.g. Sabouraud-glucose-agar, Kimmig-agar

  • subsequent microscopical examination of smears with lactophenole cotton blue 
    or with PAS-staining or Immunofluoreszence (pathohistology)

  • Differentiation between P. zopfii, P. wickerhamii  and P. blaschkeae by assimilation of Trehalose and Arginine. P. wickerhamii utilize trehalose; P. zopfii and P. blaschkea do not. P. blaschkeae can't utilize Arginine, whereas, both Genotypes of P. zopfii use this amino acid.

 

Infections:

Human protothecosis

  • only in exceptional cases, usually caused by P. wickerhamii

  • onychomycosis

 

Pathogenity

  • facultative pathogene

  • predisponating factors, e.g. immunosupression, are assumed

 

Immune response

  • induction of specific systemic and lokal antibodies of immunglobuline
    isotypes G and A

  • high resistance to phagozytosis 

  • Persistence and proliferation within macrophages and PMN's